Electrophoresis Gel Bands

This is a picture of a gel from the current production run of N6 Vector B. We separate the stock plasmid DNA into multiple bands via electrophoresis on a 1% agarose gel. Using the IORodeo transilluminator we visualize the DNA bands, and use a scalpel to slice off the correct band of plasmid DNA. Then, we run the slices through an agarose gel extraction kit and an endotoxin purification kit, before sending them off for Sanger sequencing and endotoxin validation.

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